[PDF/Kindle] CRISPR-Cas: A Laboratory Manual by Jennifer Doudna
CRISPR-Cas: A Laboratory Manual. Jennifer Doudna
CRISPR-Cas-A-Laboratory-Manual.pdf
ISBN: 9781621821311 | 450 pages | 12 Mb
- CRISPR-Cas: A Laboratory Manual
- Jennifer Doudna
- Page: 450
- Format: pdf, ePub, fb2, mobi
- ISBN: 9781621821311
- Publisher: Cold Spring Harbor Laboratory Press
Pdf format ebooks free download CRISPR-Cas: A Laboratory Manual 9781621821311 by Jennifer Doudna in English
CRISPR-Cas-based techniques are revolutionizing the way geneticists and molecular biologists modify DNA sequences and modulate gene expression in cells and organisms. This laboratory manual presents step-by-step protocols for applying this cutting-edge technology to any system of interest. Contributors describe approaches for designing guide RNAs that precisely target the gene of interest, delivering CRISPR-Cas reagents into cells (e.g., using AAV vectors), and selecting cells that harbor the desired changes. Strategies for scaling and multiplexing these procedures are also described, as are methods for optimizing CRISPR-Cas systems in common species and cell lines, from yeast to human.
Genome engineering using the CRISPR-Cas9 - Zhang Lab @ MIT
and the RNA-guided CRISPR-Cas nuclease system18–25. The first two technologies use the Illumina user manual. Off-target indel analysis
Patent US8697359 - CRISPR-Cas systems and methods - Google
The CRISPR-Cas system of claim 15 , wherein the expression of two or and Maniatis, MOLECULAR CLONING: A LABORATORY MANUAL,
The Streptococcus thermophilus CRISPR/Cas system provides
The CRISPR/Cas adaptive immune system provides resistance against phages and plasmids in Molecular Cloning: A Laboratory Manual.
Cold Spring Harbor Lab Press Genomics
CRISPR-Cas: A Laboratory Manual Edited By Jennifer Doudna, HHMI/University of California Berkeley; Prashant Mali, University of California San Diego.
Patent EP2764103A2 - Crispr-cas systems and methods for altering
Also provided are methods of directing CRISPR complex formation in and Maniatis, MOLECULAR CLONING: A LABORATORY MANUAL,
CRISPR-Cas: A Laboratory Manual - Cold Spring Harbor Laboratory
Featuring contributions from leading figures in the CRISPR–Cas field, this laboratory manual presents a state-of-the-art guide to the technology. It includes
Crispr-Cas: A Laboratory Manual: Amazon.de: Jennifer Doudna
The development of CRISPR-Cas technology is revolutionizing biology. Based on machinery bacteria use to target foreign DNA, these powerful techniques
Adenoviral vector delivery of RNA-guided CRISPR/Cas9 nuclease
CRISPR/Cas9-derived RNA-guided nucleases (RGNs) are DNA A Laboratory Manual, 3rd Ed. Sambrook, J. F. & Russell, D. W. (eds.)
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CRISPR-Cas and restriction–modification systems are compatible
Here, we show that R-M and CRISPR-Cas systems are compatible and J. & Russell, D. W. Molecular Cloning: A Laboratory Manual 3rd edn.
Cold Spring Harbor Lab Press Books
CRISPR-Cas: A Laboratory Manual Edited By Jennifer Doudna, HHMI/University of California Berkeley; Prashant Mali, University of California San Diego.
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