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Procedure for CBC Count: Follow the procedure on the coulter machine. Procedure for manual RBC Count: Make a 1:200 Dilution using EDTA blood and saline.The decrease in red blood cell count is called anemia. Oxygen-carrying Until the 1950s, blood count remained to be a manual procedure, but type=pdf. Hemo: blood; Cyto: cell, Meter: measurement / count. c) RBC pipette d) WBC pipette. NEUBAUER'S SLIDE Thus, dilution factor for RBC counting is 200. The hemocytometer cell counting (manual method) technique was used in this study to estimate the total SVF cell count in 5 g of adipose tissue ( Figure-2) [13] Both are thoroughly mixed. Dilution factors. Page 14. Red Blood Cell Count. Methodology.