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* Use up to 2.5 U for longer targets. PCR Protocol. See page 2 to view a procedure for preparing and running your PCR experiment. Protocol: The following general procedure is suggested as a guideline and as a starting point when using PLATINUM Taq DNA Polymerase in any PCR amplification. The streamlined, easy-to-follow protocol provided with the kit simplifies PCR setup. For added convenience and easier handling, CoralLoad PCR Buffer is provided Inactivated by phenol/chloroform extraction. PROTOCOL. To prepare several parallel reactions and to minimize the possibility of pipetting errors, prepare a PCR Mg++ concentration of 1.5–2.0 mM is optimal for most PCR products generated with Taq DNA Polymerase. The final Mg++ concentration in 1X Standard Taq Reaction Total Volume. 20. * Could go down to 0.04 - 0.1 ul if needed according to invitrogen protocol. 2. Add 0.5 - 4 µl of DNA sample to each PCR tube.For country-specific contact information, visit invitrogen.com. Page 2. Page 2. Basic PCR Protocol. The following protocol serves as a general guideline Here, a protocol using TOPO TA cloning kit (Invitrogen) is shown. If screening of colonies containing plasmid with insert DNA is necessary, we recommend doing