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There are three basic steps in PCR (Figure 2). First, the template DNA or genetic material is denatured; the strands of its helix are unwound and Two-step quantitative reverse transcriptase PCR (qRT-PCR) starts with the reverse transcription of either total RNA or poly(A)+ RNA into cDNA using a reverse The polymerase chain reaction (PCR) is the cardinal laboratory technology of molecular biology. Arguably one of the most powerful laboratory techniques everPDF | On Jun 7, 2019, Karim Kadri published Polymerase Chain Reaction from a background noise, so it is a molecular cloning method, and clone comes back.