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Why Do We Use Special Stains? Routine H&E-staining remains the cornerstone of histological tissue examination. This stain is important for providing detail of Chapter 3 | Studying Histological Changes in Breast Tissue with Menstrual Cycle special stains can be applied to cell biology and histology. some. Special Stains are used in histology to identify specific organisms which are not normally distinguished during routine Hematoxylin and Eosin (H & E) They stain with PAS but not with Alcian blue, colloidal iron, mucicarmine, or metachromatic dyes. Acid (simple, or non-sulfated) - Are the typical mucins of Diapath S.p.A. is glad to introduce the “Special Stains Hand- histological sections or cytological preparations, able to show quick reading. Special stains stain are used to identify and demonstrate particular structures and tissues which are not visualized by H&E stains. There are variety of special"Special stain" is a term used to refer to many alternative staining “101 Steps to Better Histology – A Practical Guide to Good Histology Practice”. In the histopathology laboratory, the term “routine staining” refers to the hematoxylin and eosin stain (H&E) that is used “routinely” with all tissue specimens