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2.2 Experimental Design Considerations for qPCR Real-Time qPCR Data Analysis 7.3 GM Soy Detection Using a Singleplex SYBR Green I qPCR Assay.PDF | Real-time quantitative PCR allows the sensitive, specific and reproducible quantitation of nucleic acids. Since its introduction, real-time. Converting RNA to cDNA with the AffinityScript QPCR cDNA Synthesis abrf.org/ResearchGroups/NucleicAcids/Studies/Assaydesign-Primer3.pdf. The polymerase chain reaction (PCR) is one of the most powerful technologies in molecular biology. Using PCR, specific sequences within a DNA or cDNA