Manual cell counting

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Manual cell counting offers an accessible way to determine the concentration of cells in a liquid sample, requiring just a light microscope and hemocytometer. Manual cell counts in a hemocytometer. Counting of cells (eukaryotic and especially mammalian cells) in a hemocytometer is an old but still valuable method to 1. Delicate procedure of coverslip preparation · 2. Manual errors counting the cells · 3. Cell count variability · 4. Inconsistent live/dead counts · 5. Challenging Viability · If the cell counts for each of the 16 squares were 50, 40, 45, 52, the average cell count would be: · (50 + 40 + 45 +52) ? 4 = 46.75 · 46.75 x 10,000 (