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For more information, please refer to the handbook “Gel filtration, Principles &. Methods”, which can be ordered from GE Healthcare, or the “Method Handbook This handbook describes the use of size exclusion chromatography (SEC) for the purification and separation of biomolecules, with focus on practical This handbook focuses on the most up-to-date SEC resins and prepacked columns from GE Healthcare Life Sciences. The resins available, selection criteria, and. Handbook. 18-1132-29. Ion Exchange Chromatography. Principles and Methods. 18-1114-21 GF: gel filtration (sometimes referred to as SEC: size exclusion Size exclusion chromatography (SEC) separates molecules based on their size by filtration through a gel. The gel consists of spherical beads containing GE Healthcare. Desalting/buffer exchange and size exclusion chromatography other SEC resins are explained in more detail in the handbook. Size ExclusionCalibration of a gel filtration column (HiLoad16/60 column, Superdex200 matrix, GE; Exemplified chromatogram of a separation of a purified scFv antibody Handbooks from GE Healthcare Life Sciences. For more information refer to gelifesciences.com/ Size exclusion chromatography (SEC) in practice . For more information, please refer to the handbook Size exclusion chromatography, Principles and Methods, which can be ordered from GE Healthcare or