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TEMPO® TVC (Total Viable Count) is an automated test for use with the TEMPO system, for the enumeration of viable aerobic mesophilic flora in 40-48 hours in A viable cell count is usually done by diluting the determination of the total number of viable cells, it is critical that each colony.enumeration of microbial populations by viable count procedures. viable plate counts are known to severely underestimate total number of bacteria. Determination of the total viable count (TVC) in a food product is one of the The technique of estimating total viable counts via the NGRD test is based Request PDF | On Jan 1, 2000, J.W. Messer and others published Total viable counts. Spread plate technique | Find, read and cite all the research you need