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If you need ultra-pure plasmid. DNA for automated or manual sequencing, we recommend the S.N.A.P.™ MiniPrep. Kit (Catalog no. K1900-01). 3. Analyze the plasmids Cloning kit contains comp cells, optimized 2X Cloning Master Mix, a ligation enhancer + linearized vector with a novel mechanism for low background. A.0 Introduction Follow these instructions to use the TOPO® TA Cloning® Kit to clone your PCR product into the TOPO® vector and transform.TOPO® TA Cloning® Kit. Five-minute cloning of Taq polymerase-amplified PCR products. Catalog numbers (pCR™2.1-TOPO® vector) K4500-01, K4500-40, K4500-J10, TOPO TA Cloning® Kits are available with either pCR®2.1-TOPO® or pCR®II-TOPO DNA for automated or manual sequencing, we recommend the S.N.A.P.™ MiniPrep. I am using a TOPO-TA cloning kit from Thermo Fisher (K4575J10) for cloning. reaction and transformation is performed according to manufacturer's manual. Among the methods for cloning PCR products with. 3'-A overhangs, the TA cloning method utilizes a T-vector with a single deoxyribothymidine (dT) addition at the