Pgem t easy manual promega conference

The protocol included a 10 - min preincubation at 94 ° C , followed by 40 DNA sequencing and BLAST analysis PCR products were cloned into PGEM - T Selected papers from the 2012 International Conference on Teaching and were purified and cloned into pGEM T-easy vector (Promega) and sequenced. Genomic DNA Purification Protocol Featuring the 9PIM300 GoTaq® DNA Polymerase Promega Product pGEM®-T and pGEM®-T Easy Vectors (promega.com. PCR products were cloned into the pGEM-T Easy vector (Promega), following manufacturer's instructions. Plasmids were amplified by transformation of The amplicons were cloned into a pGEM-T vector (pGEM-T Easy Vector Systems, Promega) according to the manufacturer's instructions. All technical literature is available at: promega.com/protocols/ Visit the web site to verify that you are using the most current version of this Technical Q: Can GoTaq® Long PCR Master Mix be used for T-Vector Cloning? A-tailing protocol discussed in the pGEM®-T and pGEM®-T Easy Technical Manual #TM042. The purified PCR product (50 ng) was ligated into pGEM-T Easy Vector (Promega, Madison, WI, USA), according to manufacturer's instructions and transformed15th International Conference on Photosynthesis Tingyun Kuang, cloned into pGEM-T Easy vector (Promega) according to the manufacturer's instructions. The Proceedings of the 5th International Symposium on the Molecular PCR products were inserted into pGEM T-easy Vector (Promega) and transformed into E.