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Here, a basic, straight-forward PCR protocol is presented. Where appropriate, some of the choices presented in the Polymerase Chain Reaction Tutorial. The purpose of PROTOCOL 2 is to amplify human DNA for downstream protocols. STUDENT LEARNING GOALS. 1. Practice essential molecular biology techniques.Products for Manual and Automated Isolation of Nucleic Acids. A basic set of PCR (chapter 4) and RT-PCR (chapter 5) protocols, including tips on. The Polymerase chain reaction (PCR), first envisaged in 1984 by Kary Mullis, has revolutionized life sciences and has become an essential technique in many The Polymerase Chain Reaction (PCR) is a powerful and sensitive technique for DNA amplification (1). Taq DNA Polymerase is an enzyme widely used in PCR (2). This is a basic PCR protocol using Taq DNA polymerase. Find additional protocols for other polymerases or advanced PCR techniques in the Protocols section