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PDF | Thin layer chromatography (TLC) is a quick, sensitive, and inexpensive technique used to determine the number of components in a mixture, verify. Thin-layer chromatography (TLC) is an extremely valuable analytical technique in the organic lab. It provides a rapid separation of compounds, and thereby givesAnalysis of Carbohydrates by Thin-Layer Chromatography. 47. A Summary of the Use and Chemistry of Carbohydrate Binders. 48. Analytical Methodology. Thin layer chromatography (TLC) is a useful technique for the separation and identification of compounds in mixtures. TLC is used routinely to follow the Line 1 cm from the bottom with a pencil as a part should be spotted. 2. TLC Plate Preparation. Page 13. 3.Spotting' TLC plates. Thin layer chromatography, or TLC, is a method for analyzing mixtures by separating the compounds in the mixture. TLC can be used to help determine the