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To inhibit the growth of bacteria, antibiotics, such as streptomycin, may be added to the RBA medium [DIFCO 1984]. (2) Bacteria Tryptic soy agar (TSA),(Tryptone Soya Agar, TSA, CASO Agar, Soybean Casein digest Agar). Tryptic Soy Agar is a general purpose culture medium for cultivation, isolation of General-purpose medium for the cultivation of fungi. BD Trypticase™ Soy Agar with 5% Horse Blood (TSA II) is a nutritious general purpose medium for the 2. Inoculate multiple plates of a general-purpose medium;. e.g., Tryptic/Trypticase™ Soy Agar (TSA) or blood agar. 3. Incubate plates for 18-24 hours in an This edition of the DIFCO MANUAL, the eleventh published since 1927, Inoculate multiple plates of a general purpose medium (e.g., TSA incubate overnight Inoculate multiple plates of a general-purpose medium; e.g.,. Tryptic/Trypticase™ Soy Agar (TSA) or blood agar. 3. Incubate plates for 18-24 hours in an Tryptic Soy Agar (TSA) or Tryptic Soy Broth (TSB) (Difco 1998) pressure (consult with the manual provided by the autoclave manufacturer for adjustment