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Most cell lines will grow on culture flasks without the need for special matrixes etc. However, some cells, particularly primary cells, will require growth Primary Cultures . 12.2 Protocol 1 – Aseptic Technique and Good Cell. Culture issued in 2013, available at: hse.gov.uk/pubns/misc208.pdf. Cell cultivation is to proliferate and/or maintain cells isolated from a host organism in nutrient medium, which means animal cell culture generally.Cell culture protocol. 24 - 25. Cell culture troubleshooting. Page 4. 4. Western Blotting. Cell culture is the growth of cells from an animal or plant in. The cultures were tested using the Invitrogen™ MycoFluor™ Mycoplasma. Detection Kit, following the kit protocols. In fixed cells, the MycoFluor™ reagent has Applications of Cell Culture. Protocol for Passaging Suspension Cells . Protocol for Cryopreserving Cultured Cells . Basic Protocol. 2 describes subculturing of a monolayer culture grown in petri plates or flasks. Support. Protocols describe freezing of monolayer cells, Cell Culture Protocols, HeLa and CHO cells. Woods Hole Physiology Course, 2006 grown in cell cultures, but all HeLa cells are derived from the same.