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To stain smears using the Ziehl-Neelsen method you will need: whqlibdoc.who.int/publications/2011/9789241501606_eng.pdf. PDF | On Dec 1, 2007, P Angra and others published Ziehl-Neelsen staining: Strong red on weak blue, or weak red under strong blue? The Ziehl-Neelsen method is probably the best known and most frequently used procedure for staining tubercle bacilli. The method requires controlled heatingZiehl-Neelsen's Staining · 10 · To stain the given xed smear of sputum by Ziehl Neelsen's technique. · Ordinary aniline dye solutions do not readily penetrate Three types of staining procedures are used for the detection of acid-fast organisms: fluorochrome, Ziehl-Neelsen (hot stain), and. Kinyoun (cold stain). The