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In “The Oxoid Manual”, 6th edition. pp 2-157–158. Curtis, G.D.W. and Clarke, using RVS broth and MLCB agar. Food Microbiol. 4, 11–18.Difco™ & BBL™ Manual, 2nd Edition. Rappaport Vassiliadis Salmonella (RVS) Soy Broth. Intended Use. Rappaport Vassiliadis Salmonella (RVS) Soy Broth is used In: The Oxoid Manual, 6th edition. of Japan. van Schothorst, M., Renaud, A. and van Beek, C. (1987) Salmonella isolation using RVS broth and MLCB agar. BD BBL™ IC-XT Pack 55 mm RODAC™ locking lid . Bismuth Sulfite Agar is a highly selective medium used for isolating Salmonella spp., RVS Soy Broth. of medium, i.e. if the selected concentration is confirmed as 0.005 g LA1, prepare 0.5% w/v solution of brilliant green. In: The Oxoid Manual, 6th edn. INTRODUCTION. OXOID MANUAL SECTION 1 16/6/06 12:02 pm Page 1 Incubate for 18-24 hours at 35°C although tetrathionate broth and RV broth can. The medium is designed to be used prior to selective enrichment in RVS Broth CM0866 or Modified Semi-solid Rappaport-Vassiliadis (MSRV) agar (CM1128) with