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How to do PCR A standard polymerase chain reaction (PCR) setup consists of four steps: PCR tubes are loaded into a thermal cycler for the amplification step a clear zip-lock bag with an absorbent pad; a bag with a QR code; 3 stickers; a box. Step by step instructions. How to do a PCR test The main steps for doing a PCR test · Wash your hands with soap or use a hand sanitiser. · Lay out all the items in the test kit on the clean surface. · Blow your A protocol to extract DNA fragments 100bp to 10kb from standard or low-melt agarose gels in either Tris acetate (TAE) or Tris borate (TBE) and purify PCRPCR protocol, PCR steps · Thaw all reagents on ice. · Assemble reaction mix into 50 µL volume in a thin walled 0.2 mL PCR tubes. · Add reagents in following order: