Topo blunt ii manual

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Alt Name: pCR-Blunt II-TOPO. Analyze: Sequence. Plasmid Type: Unspecified. Cloning Method: TOPO Cloning. Size: 3519. 5' Sequencing 1 Primer: SP6. Blunt-end ligations require an excess of insert compared to your vector. 2. You also need to use a high concentration of ligase. 3. Blunt-end ligase reactionsTOPO reactions! USER GUIDE. For Research Use Only. Not for use in diagnostic 2. ?85°C to ?68°C. Zero Blunt® TOPO®. PCR Cloning Kits for Sequencing. Includes: Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector; Salt solution; dNTPs; Control template; M13 forward and reverse primers; Cloning Kit manual for instructions. 25 µg/mL Zeocin™. transformation. Plasmid Purification Kit (Catalog no. K2100-01). 2. Add 2 µL of the TOPO® Cloning The kits include linearized and topoisomerase I-activated pCR-Blunt II-TOPO Vector for 5-minute benchtop ligations witho. A.0 Follow these instructions to TOPO® Clone your blunt-end PCR product into pCR™-Blunt II-TOPO® and transform the reaction into chemically