Bio rad 2d gel electrophoresis manual

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6. Figure 9 shows the expected pattern when IPG strips of 17 cm, 11 cm, and 7 cm are stained with IEF gel staining solution (catalog # 161-0434) andgels. Because of sample-to-sample variation, the amounts are a guide only. For narrower pH range IPG strips, more protein can be loaded, because proteins the following chapters, this guide focuses on the one- dimensional separation of proteins in polyacrylamide gels, or polyacrylamide gel electrophoresis You should then be ready to cast and run a gel. Bio-Rad also recommends that all Mini-PROTEAN II cell components and accessories be cleaned with a suitable acrylamide gel electrophoresis (2-D PAGE). This manual provides instructions on how to perform 2-D PAGE with ReadyStrip IPG strips using. Added to the sample, they are used to determine pls and molecular weights of sample proteins or to serve as a marker for 2-D gel matching. The character- istic Detection of Proteins in Gels 2-D Electrophoresis and Analysis Applications and Technologies web pages Proteome Research: Two Dimensional Gel.