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Cell lines: A cell line is a permanently established cell culture that will proliferate ation_of_Stable_Cell_Lines_low_res.pdf After the first subculture, the primary cells start to become a cell line or subclone. Cell lines derived from primary cultures have a limited lifespan as a Types of Cell Lines: Finite Cell Lines: The cells in culture divide only a limited number of times, before their growth rate declines and they eventually die. Suspension cell lines often have a recommended sub-culture seeding density. Always check the guidelines for the cell line in use. Some slow growing cells mayeven greater array of cell lines, cell culture products, knowledge, and services issued in 2013, available at: hse.gov.uk/pubns/misc208.pdf. Cell lines are cultures of animal cells that can be propagated repeatedly and sometimes indefinitely. They arise from primary cell cultures. Primary cultures After the first subculture, the primary culture becomes known as a cell line or subclone. Cell lines derived from primary cultures have a limited life span ( Cell and tissue culture technology holds a central position in modern biomedical research. Cells in culture are useful model systems for studying normal