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Manual Tracking. The content of this page has not been vetted since shifting away from MediaWiki. If you'd like to help, check out the how to help guide! The "Find Maxima" tool built into ImageJ outperforms this algorithm in terms of computational performance and the restriction to true maxima. Nevertheless, I To manually track a particle use the point-tool. First double click on the point-tool, select Auto-Next Slice, Add to ROI Manager and Label Points. and press ok A bit old review on single particle tracking by Saxton is still an excellent textbook ImageJ “manual tracker plugin”, we will do this in the practical.2005/06/15: New features: 2D centring correction, Directionality check, Previous track files may be reloaded, 3D features added (retrieve z coordinates, Particle Tracker is a 2D and 3D feature point-tracking tool for the automated detection and tracking of particle trajectories as recorded by video imaging MTrackJ: An ImageJ Plugin for Motion Tracking and Analysis ImageJ users need to manually download the following two jar-files to the plugins folder.