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At small Camera Length settings, the beam may not be able to reach the STEM detector. Manual CONTRAST and BRIGHTNESS controls, as well as AUTO C/B, are located FEI Tecnai G2 F20 Operating Procedures. 1. Sign-up in the microscope into the FOM system. 2. Startup the software: Start the software in this sequence:This manual is to be used as a guide to the basic operation of the Tecnai F-20 microscope. View and Download Tecnai F20 operating instructions manual online. CSB CryoEM Facility TF20. F20 microscope pdf manual download. Preface. The Operating Instructions of the Tecnai microscope are divided into a paper part (this manual) and a digital part (the on-line help). Tecnai on-line help manual -- Modes Tecnai Imaging & Analysis . Note 1: The Tecnai microscope uses (small) image shifts between different FEI TECNAI G2 F30 TWIN TEM. Training Manual To retrieve the files from your office PC, also ask staff for the instructions. Abbreviations used in the manual: DL = Direct Alignment; Left Hand Panel = LHP; Find the “Shortcut to Tecnai F20” folder on desktop, locate your saved TF20: Tecnai G2 200kV TEM (FEI). OPERATION MANUAL for Basic TEM. The FEI Tecnai TF20 is a 200kV FEG high resolution Transmission Electron.