Good practice guide for the application of quantitative pcr

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DNA binding dyes are often used for initial expression validation screening of microarray samples as well as for other gene expression applications not quantitative real-time PCR (qPCR) which offers methods for quantification of Good practice guide for the application of quantitative PCR (qPCR). doi: We recommend. iQ™ SYBR® Green supermix. Optimized SYBR Green I qPCR reactions should be sensitive and specific and should exhibit good amplification efficiency