Plant tissue culture laboratory equipments pdf

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for maintenance of equipment and apparatus. Up-to-date record all the need of chemicals, glassware, instruments, etc. to avoid the lack of laboratory material a) Glassware washing b) Culture media preparation. Page 2. c) Media and equipment sterilization d) Ex-plants preparation and aseptic transference of cultivated Autoclave is used to sterilize medium, glassware and tools for the purpose of plant tissue culture. The same equipment is used in hospitals to sterilize gauge, plant. The whole process requires a well equipped culture laboratory and nutrient medium. This process involves various steps, viz preparation of nutrient 4 Plant Biotechnology Lab. 11. 5 Equipment : Principle and Working. 14. 6 Tabulations. 31. 7 Preparation of Solutions. 32. 8 Basics of Plant Tissue Culture.Typically, the culture room for growth of plant tissue cultures should have a temperature between 15° and 30° C, with a temperature fluctuation of less than ± propagating new plant varieties, conserving rare and endangered plants, A tissue culture laboratory must be designed to accommodate the equipment.