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Coronavirus PCR testing – Self-test instructions. Self-test instructions for taking a combined throat and nose swab for Coronavirus (COVID-19). The main steps for doing a PCR test · Wash your hands with soap or use a hand sanitiser. · Lay out all the items in the test kit on the clean surface. · Blow your PCR protocol, PCR steps · Thaw all reagents on ice. · Assemble reaction mix into 50 µL volume in a thin walled 0.2 mL PCR tubes. · Add reagents in following order: The Polymerase Chain Reaction (PCR) is a powerful and sensitive technique for DNA amplification (1). Taq DNA Polymerase is an enzyme widely used in PCR (2). The Instructions For Use rev.1.2. 1. Novel Coronavirus 2019-nCoV Nucleic Acid Detection Kit (Real Time PCR). Instructions For Use. [Product Name].How to do PCR · Add required reagents or mastermix and template to PCR tubes. · Mix and centrifuge. *Add mineral oil to prevent evaporation in a thermal cycler PCR test instructions and what to do if you have a positive, negative or 'could not be read' result.