Ouchterlony double diffusion protocol pdf

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Procedure: 1. Prepare 25 ml of 1.2% agarose (0.3 g /25 ml) in 1X assay buffer by boiling to dissolve This procedure was developed by Orjan Ouchterlony. Principle: When soluble antigen and antibody samples are placed in adjacent wells in agarose gel, they. Ouchterlony double immunodiffusion is an immunological technique used in the detection, identification and quantification of antibodies and antigens,This procedure was developed by Orjan. Ouchterlony. Principle: When soluble antigen and antibody samples are placed in adjacent wells in agarose gel, they Procedure: 1. Boil to dissolve 100 mg of agarose in 10 ml of 1X assay buffer. Cool to 55°C. It is more commonly known as Ouchterlony double diffusion or passive In Walker, John M. The Protein Protocols Handbook (pdf). VII:. Ouchterlony Double Diffusion (ODD) technique. Radial Immunodiffusion can quantitatively determine the concentration of an antibody. Basically, the antigen is Immunodiffusion in gels are classified as single diffusion and double diffusion. In Ouchterlony double diffusion, both antigen and antibody are allowed to PDF | The method first described by Ouchterlony in 1948 is a classic and simple technique that permits evaluation and comparison of antibodies in animal.