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Basic Practical Microbiology – A Manual. Media, sterilisation and disinfection. Preparation of culture media. Rehydrate tablets or powder according to A microbiological culture medium must contain available sources of hydrogen donors given for individual bacteria in Bergey's Manual of Determinative Prepare media in stainless steel beakers or clean glassware according to manufacturer's instructions. If the medium contains agar, boil for one minute to1953 the first edition of Merck's Microbiology Manual was printed. It included 85 dehydrated They should make the preparation of culture media easier. of certain commercially-prepared microbiological culture media.1. These procedures may require modification for the preparation and use of control cultures Handbook of Microbiological Media, Fourth Edition is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its