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x Preparation and sterilisation of equipment and culture media. Present minimum risk; refer to list of suitable cultures. Microbiology. Notes. 9. BACTERIAL CULTURE. MEDIA. 9.1 INTRODUCTION. Why bacteria have to be grown (cultured) in the laboratory on artificial culture media? Basal media are those that may be used for growth (culture) of bacteria that do not need enrichment of the media. Examples: Nutrient broth, nutrient agar and Liquid media are sometimes referred as “broths” (e.g nutrient broth). In liquid medium, bacteria grow uniformly producing general turbidity. Certain aerobic3. Solid medium: If agar is added to a nutrient broth, it becomes solid medium. It is used for isolating microbes and to determine. Basal media are those that may be used for growth (culture) of bacteria that do not need enrichment of the media. Examples: Nutrient broth, nutrient agar and