Manual tissue processing procedure

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Tissue processing is a procedure of removing water from cells and replacing Microwave assisted processing: might require manual transfer of specimen orOverview of the steps in tissue processing for paraffin sections · 1. Obtaining a fresh specimen · 2. Fixation · 3. Dehydration · 4. Clearing · 5. Wax infiltration. Processing: The steps in this process are dehydration and clearing. Tissue processing may be performed manually or with the help of automated. Tissue processing can be performed manually (hand processing), In this procedure, tissue is fixated, dehydrated through a series of graded ethanol baths A. Tissue processing. B. Tissue Embedding Techniques. C. Microtome / Sectioning. D. H&E staining (Leica multistainer). E. H&E staining (Manual).