Mccp download

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The strategy that was endorsed by the participants includes the control of other priority small ruminant diseases such as CCPP and Capripox. For that purpose, this one-step multiplex RT-qPCR for the identifica- tion of four pathogens responsible for respiratory diseases of small ruminants will be a useful tool for disease surveillance.

Additionally, other pathogens responsible for respiratory diseases of small ruminants need to be included in the multiplex based tests. In the meantime, prelimi- nary results obtained in this study will undergo further evaluation by testing additional sam- ples, healthy and infected, to establish it as a routine and efficient surveillance diagnostic tool.

Supporting Information S1 Table. DOC S2 Table. DOC S3 Table. DOC S4 Table. DOC S5 Table. DOC S6 Table. DOC S7 Table. Details of the DNA samples extracted from different pathological samples col- lected from Goat and Sheep showing the symptoms of respiratory infections and results on testing by one-step multiplex RT-qPCR. DOC S8 Table. Details of the DNA samples extracted from different pathological samples col- lected from cattle showing the symptoms of respiratory infections and results on testing by one-step multiplex RT-qPCR.

DOC S9 Table. Performance of the multiplex assay in detection of TNA of different targets represented by Cq values extracted using two different extraction kits. DOC S11 Table. Comparison of performance of multiplex assay for detection of PPRV with two previously described methods. DOC Acknowledgments The authors duly acknowledge all the veterinarians and technicians involved in the collection and provision of samples used during the development and validation of the assay.

The authors would also like to acknowledge Dr Jenna Achenbach for language editing of the manuscript. References 1. Trop Anim Health Prod — J Vet Diagn Invest — OIE, France. Brown C. Small Ruminant Research 21— Transbound Emerg Dis — Animal Frontiers 4: 14— Journal of general virology — FEMS microbiology letters 1— PMID: Archives of Razi 47— Small Ruminant Research — Thiaucourt F, Bolske G.

Revue scientifique et technique International Office of Epizootics — Vet Res Journal of Zoo and Wildlife Medicine 93— Vet J — BMC Vet Res 8: 37 doi: Venkatesan G, Balamurugan V, Bhanuprakash V Multiplex PCR for simultaneous detection and differentiation of sheeppox, goatpox and orf viruses from clinical samples of sheep and goats.

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