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Pereira VC 1 ,. Riboli DF 1 ,. Affiliations 3 authors 1. Share this article Share with email Share with twitter Share with linkedin Share with facebook. Abstract Background Methicillin-resistant Staphylococcus aureus MRSA are important pathogens in neonatal and pediatric intensive care units, which can cause severe infections in hospitalized children.

Detection of the mecA gene and classification of the staphylococcal cassette chromosome mec SCCmec permit the characterization of MRSA strains isolated from infections caused by these microorganisms. Results The mecA gene was detected in Conclusions Replacement of clonal groups occurred in the neonatal and pediatric units over the period studied, a fact highlighting the importance of improving hygiene practices and control measures of nosocomial infections in these units.

Free full text. Ann Clin Microbiol Antimicrob. Published online Nov 7. PMID: Author information Article notes Copyright and License information Disclaimer. Corresponding author. Received May 14; Accepted Oct This article has been cited by other articles in PMC. Go to:. Background Methicillin-resistant Staphylococcus aureus MRSA are important pathogens in neonatal and pediatric intensive care units, which can cause severe infections in hospitalized children.

Methods A total of S. Results The mec A gene was detected in Strains A total of S. DNA extraction Total nucleic acid was extracted from S.

Detection of the mec A gene The mec A gene was investigated in the S. Pulsed-field gel electrophoresis The clonal profile of the Staphylococcus spp. Open in a separate window. N: number of strains. Characterization of the staphylococcal cassette chromosome mec The 21 mec A gene-positive S. Evolution of oxacillin resistance in S. Figure 1. Competing interests The authors declare that they have no competing interests.

New York: Springer Verlag; Taxonomic Outline of the Procaryotic Genera. Bergdoll MS. Staphylococcus aureus. J Assoc Off Anal Chem. Bannerman TL. Staphylococcus , Micrococcus and other catalase-positive cocci that grow aerobically. Manual of Clinical Microbiology.

Prevalence of Staphylococcus aureus nasal colonization in the United States, — J Infect Dis. Comparison of community-acquired methicillin-resistant Staphylococcus aureus bacteremia to other staphylococcal species in a neonatal intensive care unit. Eur J Pediatrics. Archer G, Niemeyer DM. Origin and evolution of DNA associated with resistance to methicillin in Staphylococci. Trends in Microbiol. Antimicrob Agents Chemother. Deresinski S. Methicillin-resistant Staphylococcus aureus : an evolutionary, epidemiologic, and therapeutic odyssey.

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