Ap biology lab manual lab 5

Note: you will be using handouts from Carolina Biological kits for this laboratory procedure. Figure 8 is the plasmid we will be using to transform.

Inside the circular plasmid there are two bold, thick black. These are the two genes on this plasmid that we are interested in. What are they? Transformation: Starter Plates of E. The plasmid used is pBLU which has a gene for ampicillin resistance and a gene which will cause a. In a controlled experiment, the E. The cells will be spread on luria broth agar with and without ampicillin for Gel Electrophoresis: A gel casting tray will be sealed on each end with masking tape, liquid agar will be poured into.

The comb will be removed. The box will be covered with wrap. The next morning, micropipets will be used to transfer DNA subjected to various restriction enzymes into the wells,. Design data tables that will enable you to collect qualitative and quantitative data from both procedures:.

The questions will be found on student guides from the Carolina Biological kits for these labs. You will need to construct a graph in order to determine the number of base pairs for the fragments with the EcoRI. The graph paper used is semilog graph paper. Directions will be reviewed during class. The directions are on the.

Inquiry: How was the transformation experiment controlled? Was biotechnology demonstrated? Use the transformation lab results to explain how plasmids can be used in genetic engineering. What is a restriction site? How could a mutation that alters a restriction site be detected by gel electrophoresis?

Part 1: Title Develop a title in the form of a question after completing the pre-lab. Part 2: Objectives What are the objectives for this laboratory? Describe E. Ap Biology Lab Manual Pdf 2.

Colleges may require students to present their laboratory materials from AP science courses before granting college credit for laboratory, so students are encouraged to retain their laboratory notebooks, reports, and other materials. AP Students.

AP Biology Lab Manual. In each investigation, you will find the following: Background information Tips for designing and conducting investigations Lists of materials and supplies Methods of analyzing and evaluating your results Means of communicating your results and conclusions Suggestions for additional investigative activities Copies of this book may be ordered online through the College Board Store.

Lab Notebooks and College Credit Colleges may require students to present their laboratory materials from AP science courses before granting college credit for laboratory, so students are encouraged to retain their laboratory notebooks, reports, and other materials.